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KMID : 0363320050260020398
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Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 2 p.398 ~ p.408
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Effects of Sipyukmiyukieum on DNA Synthesis, cAMP Synthesis and MHC-class II Expression of FRTL-5 Thyroid Cells
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Lee Jae-Eun
Kim Byoung-Woo
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Abstract
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Objective: Graves’ disease encompasses hyperthyroidism and diffused goiter associated with auto-antibodies to the thyroid stimulating hormone(TSH) receptors. In clinical environment, treatments of Graves’ disease have many side effects such as recurrence and hypothyroidism. We’ve studied the effects of Sipyukmiyukieum on DNA synthesis, cAMP synthesis, and MHC-class II expression of FRTL-5 thyroid cells were studied.
Methods: DNA synthesis was investigated by using BrdU staining and cAMP synthesis by ELISA kit, and expression of interferon-{gamma} activated MHC class II by Flow cytometer.
Results: After introduction of Sipyukmiyukieum, significant inhibition of DNA synthesis. cAMP synthesis, and expression of interferon-{gamma} activated MHC class II of FRTL-5 thyroid cells was observed.
Conclusions: Judging from these results, Sipyukmiyukieum has potential as a potent herbal treatment for inhibiting the enlargement of goiter, synthesis of abnormal thyroidal hormones, and autoimmuine responses of Graves’ disease.
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KEYWORD
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Sipyukmiyukieum(Shiliumeiliuqiyin), FTRL-5 Thyroid Cells, DNA Synthesis, cAMP Synthesis, MHC class II Expression.t
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